2024.04? ? 至 今：中國(guó)科學(xué)院廣州生物醫(yī)藥與健康研究院? 高級(jí)實(shí)驗(yàn)師? ? ?

2011.03--2024.03：中國(guó)科學(xué)院廣州生物醫(yī)藥與健康研究院? 助理研究員

2009.01--2011.02：廈門大學(xué)附屬演武醫(yī)院中心實(shí)驗(yàn)室? ? ?實(shí)驗(yàn)師

2003.07--2008.12：山東盛能奶牛高效繁育研發(fā)基地? ?助理實(shí)驗(yàn)師

1999.09--2003.07:? 河南農(nóng)業(yè)大學(xué)? ?動(dòng)物醫(yī)學(xué)系? ? ?學(xué)士

1.動(dòng)物克隆及基因編輯動(dòng)物?

2.顯微操作技術(shù)應(yīng)用

第一作者論文

1. Yanhui Liang#, Jingke Xie#, Quanjun Zhang#, Xiaomin Wang#, Shixue Gou, Lihui Lin, Tao Chen, Weikai Ge, Zhenpeng Zhuang, Meng Lian, Fangbing Chen, Nan Li, Zhen Ouyang, Chengdan Lai, Xiaoyi Liu, Lei Li, Yinghua Ye, Han Wu, Kepin Wang*, and Liangxue Lai*. AGBE: a dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns. Nucleic Acids Research. 2022 May 20; 50(9): 5384–5399.?

2. Quanmei Yan#, Quanjun Zhang#, Huaqiang Yang, Qingjian Zou,Chengcheng Tang,Nana Fan*,Liangxue Lai*. Generation of multi-gene knockout rabbits using the Cas9/gRNA system. Cell Regeneration. 2014 Sep 27;3(1):12.

非第一作者論文

1. Jingke Xie#, Zhenpeng Zhuang#, Shixue Gou#, Quanjun Zhang, Xia Wang,Ting Lan , Meng Lian , Nan Li , Yanhui Liang , Zhen Ouyang , Yinghua Ye , Han Wu , Liangxue Lai* , Kepin Wang* . Precise genome editing of the Kozak sequence enables bidirectional and quantitative modulation of protein translation to anticipated levels without affecting transcription. Nucleic Acids Research. 2023 Oct 13;51(18): 10075–10093.?

2. Nan Li#,Shixue Gou#, Jiaowei Wang#, Quanjun Zhang, Xingyun Huang, Jingke Xie, Li Li, Qin Jin, Zhen Ouyang, Fangbing Chen, Weikai Ge, Hui Shi, Yanhui Liang, Zhenpeng Zhuang, Xiaozhu Zhao, Meng Lian,Yinghua Ye, Longquan Quan, Han Wu*, Liangxue Lai*, Kepin Wang*. CRISPR/Cas9-mediated gene correction in newborn rabbits with hereditary tyrosinemia type I. Molecular Therapy.2021 Mar,3; 29(3): 1001-1015.

3. Zhiquan Liu # , Siyu Chen # , Huanhuan Shan , Quanjun Zhang , Mao Chen , Liangxue Lai* , Zhanjun Li* . Efficient and precise base editing in rabbits using human APOBEC3A-nCas9 fusions. Cell Discovery.2019 Jun 11;5:31.?

4. Yanling Zhu#, Zhishuai Zhang#, Nana Fan#, Ke Huang, Hao Li, Jiaming Gu, Quanjun Zhang, Zhen Ouyang, Tian Zhang, Jun Tang, Yanqi Zhang, Yangyang Suo, Chengdan Lai, Jiaowei Wang, Junwei Wang, Yongli Shan, Mingquan Wang, Qianyu Chen, Tiancheng Zhou, Liangxue Lai*，Guangjin Pan*. Generating functional cells through enhanced interspecies chimerism with human pluripotent stem cells. Stem Cell Reports. 2022 May 10; 17(5): 1059–1069.?

5.? Jingke Xie#; Weikai Ge#; Nan Li#; Qishuai Liu#; Fangbing Chen; Xiaoyu Yang, Xingyun Huang; Zhen Ouyang; Quanjun Zhang; Yu Zhao; Zhaoming Liu; Shixue Gou; Han Wu; Chengdan Lai; Nana Fan; Qin Jin; Hui Shi; Yanhui Liang; Ting Lan; Longquan Quan; Xiaoping Li; Kepin Wang*; Liangxue Lai*; Efficient base editing for multiple genes/loci in pigs using base editors. Nature Communications. 2019,June, 10(1): 2852.

6.? Li L, Zhang Q, Yang H, Zou Q, Lai C, Zhao Ping, Luo Z, Yang J, Chen Q, Wang Y, Newsome P, Frampton J, Maxwell P. H., Siu T, Tam S, Tse H, Qin B, Bao X, Esteban M*, and Lai L*. Fumarylacetoacetate hydrolase knockout rabbit model for hereditary tyrosinemia type 1. J Biol Chem. 2017 Mar 17;292(11):4755-4763.

7. Wei S#, Zou Q#, Lai S#, Zhang Q, Li L, Yan Q, Zhou X, Zhong H, Lai L. Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators. Scientific Reports. 2016 Jan 19;6:19648.

8. Song J#, Zhong J#, Guo X#, Chen Y, Zou Q, Huang J, Li X, Zhang Q, Jiang Z, Tang C, Yang H, Liu T, Li P, Pei D*, Lai L*. Generation of RAG 1- and 2-deficient rabbits by embryo microinjection of TALENs. Cell Research. 2013 Aug;23(8):1059-62.